LymPHOS is a continuously evolving PhosphoProteome DataBase for human T-Lymphocytes. And it is also a Web-Application that allows everyone to freely access all this information from the Internet.
Protein phosphorylation-dephosphorylation events play a primordial role in almost all aspects of cell function including signal transduction, cell cycle and apoptosis. Phosphorylation is probably the most frequent reversible posttranslational modification of proteins (about 30% of the cell proteins are phosphorylated in a given condition). However, the number of known in vivo substrates of kinases is still low.
Where does LymPHOS come from?
LymPHOS data has been obtained at the CSIC/UAB Proteomics Laboratory of the Spanish National Research Council (CSIC), mainly under a Spanish Research Project funded by Research and Education Ministry (BIO2004-01788) during the period 2004-2011. In this project we study, using a proteomic approach, the phosphoproteome of T and B lymphocytes upon (physiological or pharmacological)activation. The main objective was the design of a coherent and comprehensive database of phosphoproteins and phosphorylation sites in these cells. The characterization of phosphorylation motifs will help in the search for new, physiologically relevant kinases as well as in the understanding of the mechanisms involved in the regulation of protein function. This information is the first step towards the design of new therapies based on drugs controlling either kinase/phosphatase activities or the activity of other proteins in key points of the affected pathways.
- Nguyen, TD., Vidal-Cortes, O., Gallardo, Ó., Abian, J., Carrascal, M., LymPHOS 2.0: an update of a phosphosite database of primary human T cells. Database 2015, 2015.
- Carrascal, M., Ovelleiro, D., Casas, V., Gay, M., Abian, J., Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment. J. Proteome Res. 2008, 7, 5167-5176.
- Ovelleiro, D., Carrascal, M., Casas, V., Abian, J., LymPHOS: design of a phosphosite database of primary human T cells. Proteomics 2009, 9, 3741–3751.
- Gallardo, Ó., Ovelleiro, D., Gay, M., Carrascal, M., Abian, J., A collection of open source applications for mass spectrometry data mining. Proteomics 2014, 20, 2275-2279.
This work has been carried out at CSIC/UAB Proteomics Laboratory, part of the Spanish National Research Council (Consejo Superior de Investigaciones Científicas - CSIC), from Catalonia (Spain), and part of ProteoRed (Proteomics National NetWork Platform).
The people who have participated directly in the current work are:
- Mass Spectrometry, study design and implementation: Montserrat Carrascal, Nguyen TienDung and Oriol Vidal-Cortes
- Sample preparation: Montserrat Carrascal, Nguyen TienDung, Vanessa Casas and Oriol Vidal-Cortes
- Data analysis, bioinformatics and informatics: Joaquin Abian and Óscar Gallardo
- Direction: Joaquin Abian
- Marina Gay
- David Ovelleiro
Laboratorio de Proteomica CSIC/UAB
Facultad de Medicina, Edificio M, Campus UAB
08193, Cerdanyola (Barcelona), Spain
Phone number: +34 93 581 48 55